Abstract
Mycobacteria constitute vast population of bacteria that are found in animals, plants and outer environments. Tuberculosis mycobacteria are a group that causes very dangerous diseases for human and animals. Nontuberculous mycobacteria are mainly environmental and are pathogenic in certain circumstances. There are reports of finding mycobacteria in plant tissues, which can be effective to survey in case of different infectious disease issues. In a previous study, we found mycobacteria strains in alfalfa vascular tissues and identified them to species level using 16S rRNA gene sequence analysis, causing some symptoms in plant tissues and aerial parts. As these rapidly growing mycobacteria (RGM) have the potential to cause opportunistic infections in human tissues, one of the most important issues for dealing with these agents is to find the best and most effective drug to treat them. Here, we conducted some assays to investigate the MIC of 7 antibiotics and two antibacterial agents against eight Mycobacterium frederiksbergense alfalfa isolated strains, in order to find the susceptible and resistant ones. We performed susceptibility testing based on broth microdilution method using Mueller hinton broth as the medium used. Results were different among the strains and different resistant and susceptible reactions were observed among different strains against each drug tested, but ethambutol resulted in similar high MIC for all of them. This variability could be attributed to many factors, such as plant tissue composition and developmental stage from which the strains have been isolated, existence and nature of drug targets and other factors such as test conditions and environmental causes. These results can bring us some insights into design and develop new drugs for treating these kinds of organisms, based on the variations in susceptibility reactions and the plant composition effect on them.
Highlights
Mycobacteria are a group of bacteria that some of their species are very pathogenic to human and some animals
We identified some isolates of mycobacteria in alfalfa vascular tissues and some symptoms were observed in stem vascular inner tissues of alfalfa following Mycobacterium inoculation which should be practiced more for confirmation of these observations
Results were examined visually based on the turbidity of bacterial cells in each column and the well without visual growth in each column was regarded as the MIC of each compound against mycobacterial isolates
Summary
Mycobacteria are a group of bacteria that some of their species are very pathogenic to human and some animals. This genus of bacteria is divided into different categories, Tuberculosis mycobacteria which are very slow growing and nontuberculous which are slow and rapidly growing. There are some reports of finding rapidly growing mycobacteria or RGM in plant species. Other reports are as interference of Mycobacterium species with growth of Scots pine seedlings [5] and finding mycobacteria as hidden endophytes in shoot of rock plant [6]. New drugs are urgently needed to treat infections due to rapidly growing mycobacteria. Tigecycline and to a less extent linezolid have been shown to have some in vitro activity against rapidly growing mycobacteria [8] [9]
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