Susceptibility Test of Two Ca2+-ATPase Conformers to Denaturants and Polyols to Outline Their Structural Difference

Abstract

To determine the effect of denaturants [guanidine hydrochloride (GdnHCl) and urea] and polyols [with various molecular masses (62.1-600)] on calcium binding at the two hypothesized conformers (A and B forms) of the chemically equivalent sarcoplasmic reticulum Ca(2+)-ATPase, which bind two calcium ions in different manners, we examined the effect of these reagents on the calcium dependence of ATP-supported phosphorylation of the ATPase molecules and of their calcium-activated, acetyl phosphatate hydrolytic activity. (1) GdnHCl (~0.05M) and urea (~0.5M) increased the apparent calcium affinity (K (0.5)) of 2-6μM of noncooperative binding [Hill coefficient (n (H))~1] of the A form to 10-40μM. (2) The employed polyols transformed the binding of the A form into cooperative binding (n (H)~2), accompanying the approach of its K (0.5) value to that (K (0.5)=0.04-0.2μM) of the cooperative binding (n (H)~2) of the B form; the transition concentration (0.025-2M) of the polyols, above which such transformation occurs, was in inverse relation to their molecular mass. (3) The binding of the B form was resistant to these denaturants and polyols. Based on these data, a structural model of the two forms, calcium-binding domains of which are loosely and compactly folded, is presented.