Susceptibility patterns of bacteria isolated from the hospital environment towards disinfectants commonly used for surfaces and medical devices

Samira Jaouhar,Khadija Bekhti,Ikrame Zeouk,Abdelhakim El Ouali Lalami,Jawad Bouzid,S Bourekkadi,K Slimani,A Soulaymani,H Hami,A Mokhtari

doi:10.1051/e3sconf/202131901081

Abstract

This study aimed to evaluate the bactericidal activity of common disinfectants used for surfaces and medical devices. Sodium hypochlorite (D1), disinfectant (D2) composed of N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine, chloride de didecyldimethylammonium, and disinfectant (D3) composed of Didecyldimethylammonium chloride and Polyhexamethylene biguanide hydrochloride, were tested against 15 strains isolated from the hospital environment and four reference bacteria. The microdilution method was performed to assess antimicrobial activity. The susceptibility was evaluated by comparing the minimum inhibitory dilution with the dilution of disinfectant recommended by the manufacture. D1 and D2 were active against Staphylococcus epidermidis, Staphylococcus saprophyticus, Enterobacter cloacae, Escherichia coli, Pseudomonas fluorescens, Methicillin-resistant Staphylococcus aureus, Bacillus spp, Corynebacterium spp, Gram-positive bacillus, Escherichia coli ATCC 25922, Bacillus subtilis ATCC 3366, and Pseudomonas aeruginosa ATCC 27853 strains but not active against Micrococcus spp, and Staphylococcus aureus ATCC 29213. D3 was ineffective against Micrococcus spp, Bacillus Gram Positive, Staphylococcus epidermidis, and Escherichia coli ATCC 25922. Therefore, D1 and D2 can eliminate most pathogenic bacteria in hospitals, in comparison to D3. It is necessary to monitor the antibacterial activity of disinfectants against reference strains but also against those usually present on surfaces. The obtained results could have promising applications in controlling the emergence of nosocomial infections.

Highlights

disinfectant (bleach) 12° (D1) and D2 were active against Staphylococcus epidermidis, Staphylococcus saprophyticus, Enterobacter cloacae, Escherichia coli, Pseudomonas fluorescens, Methicillin-resistant Staphylococcus aureus, Bacillus spp, Corynebacterium spp, Gram-positive bacillus, Escherichia coli ATCC 25922, Bacillus subtilis ATCC 3366, and Pseudomonas aeruginosa ATCC 27853 strains but not active against Micrococcus spp, and Staphylococcus aureus ATCC 29213
The hospital environment, especially surfaces, is a source of numerous microorganisms that remains not necessarily pathogens, but some, such as those derived from the cutaneous-mucous flora of humans or the environment may be responsible for Health-care Associated Infections (HAIS) [1]
We studied the susceptibility of bacterial isolated from the hospital environment as well as reference strains against three disinfectants (D1, D2, and D3) containing various active molecules

Summary

Introduction

The hospital environment, especially surfaces, is a source of numerous microorganisms that remains not necessarily pathogens, but some, such as those derived from the cutaneous-mucous flora of humans or the environment may be responsible for Health-care Associated Infections (HAIS) [1]. Weber et al [2] reported that 20% to 40% of HAIS have been attributed to cross-infection via the caregiver’s hands contaminated by contact with the patient or contact with hospital surfaces. Adequate measures are necessary to Subsequently, surfaces and medical equipment must be cleaned and disinfected regularly several times per day in some services (operating room, burn unit, and intensive care) to reduce the risk associated with. HAI [9, 10] In this context, disinfectants are used in hospitals for many applications on surfaces and equipment.

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