Susceptibility of Helicoverpa armigera to two Bt toxins, Cry1Ac and Cry2Ab, in northwestern China: toward developing an IRM strategy

Abstract

Extensive unofficial planting of Bt cotton expressing Cry1Ac toxin has occured for the past two decades in northwestern China, and no mandatory refuge policy has been adopted. The status of Cry1Ac susceptibility of Helicoverpa armigera in this region has not been routinely monitored, nor has the susceptibility to Cry2Ab cotton which has not been released in China. The susceptibility of H. armigera populations to both toxins was assessed in 2014 and 2015 in two contrasting cotton farming systems across the region. Over the 2 years, the response to Cry1Ac of the nine H. armigera field populations sampled ranged from 3.16 to 16.94 μg ml−1 for LC50 and 0.013 to 0.741 μg ml−1 for IC50, and the baseline susceptibility of these strains to Cry2Ab ranged from 3.43 to 19.05 μg ml−1 for LC50 and 0.16 to 3.81 μg ml−1 for IC50. There was no significant difference in susceptibility to either Cry1Ac or Cry2Ab between small-holder and broad-acre farming. The susceptibility to Cry1Ac toxin in northwestern China is higher than that in northern China, while there was no difference for Cry2Ab between northwestern China and northern China. With high levels of adoption of Bt cotton and relatively limited natural refuge for H. armigera, it is important to consider resistance management measures for Bt cotton in northwestern China.