Abstract
The results of assessing the effect of Bacillus thuringiensis ssp. aizawai strain on fi ve species of lepidopteran pests (Lepidoptera) belonging to different families are presented. The mortality of larvae infected with B. thuringiensis strain varied signifi cantly depending on the species of insect. In four species: the cabbage white butterfl y (Pieris brassicae L.), the cabbage moth (Mamestra brassicae L.), the greater wax moth (Galleria melonella L.) and the black-veined white (Aporia crataegi L.), death of individuals was noted on the second day of the experiment. However, mortality rate was signifi cantly different between species and increased over time. The diamondback moth (Plutella xylostella L.) was more susceptible to the pathogen. Signifi cant mortality of the larvae of this phytophage was observed already on the fi rst day after infection; while on the second day of the experiment all individuals died. Lethal time LT90 of more resistant species (black-veined white) and more sensitive ones (diamondback moth) differed sevenfold. Semi-lethal concentrations of B. thuringiensis strain for the tested insect species were determined: on the third day of the experiment, lethal concentration LC50 for the cabbage moth larvae, the greater wax moth and blackveined white ranged from 1.7 to 4.5 × 108 spores/ ml. For the cabbage white butterfl y, it was much lower (1.8×107 spores/ml). For diamondback moth caterpillars, LC50 was 4.25×107 spores/ml already one day after infection. It was noted that the specifi city of B. thuringiensis strain is associated with various mechanisms of insect protection from pathogens, and is due to individual characteristics of the species. In particular, the pH of excrement of intact caterpillars of the cabbage white butterfl y, a species sensitive to B. thuringiensis, was 2 times higher than that of caterpillars of the greater wax moth – 8.9 and 4.3, respectively, which is one of the reasons for the susceptibility (or resistance) of the species to bacterial entomopathogen.
Highlights
For citation: Andreeva I.V., Shatalova E.I., Kalmykova G.V., Akulova N.I., Ulyanova E.G
The results of assessing the effect of Bacillus thuringiensis ssp. aizawai strain on five species of lepidopteran pests (Lepidoptera) belonging to different families are presented
The mortality of larvae infected with B. thuringiensis strain varied significantly depending on the species of insect
Summary
Восприимчивость разных видов чешуекрылых насекомых к штамму Bacillus thuringiensis ssp. Aizawai, штамм, Lepidoptera, фитофаг, энтомопатоген, рН кишечника, ЛК one day after infection. Aizawai, strain, Lepidoptera, phytophage, entomopathogen, intestinal pH, LC50. За последние 5 лет (2015– 2019) в условиях Западной Сибири массовое размножение этого вредителя на капустовых культурах отмечено в четырех вегетационных периодах, при этом аномальными по численности моли были 2015 и 2019 гг. (Lepidoptera: Noctuidae) и капустная белянка – Pieris brassicae L. Из биологических средств наибольшее распространение для контроля численности чешуекрылых вредителей в мировой практике получили биопрепараты на основе бактерий Bacillus thuringiensis (Bt). Что гусеницы капустной моли, устойчивые к Bt ssp. Ведущие мировые производители биологических средств защиты растений предлагают инсектициды на основе штаммов B. thuringiensis ssp. Ведущие мировые производители биологических средств защиты растений предлагают инсектициды на основе штаммов B. thuringiensis ssp. kurstaki и B. thuringiensis ssp. aizawai, различающихся по спектру син-
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
