Abstract

The sensitivity of various host systems to Murray Valley encephalitis virus (MVE), Sindbis virus and Getah virus was compared using viruses passaged in mice and chick embryos. The methods used were intracerebral inoculation of suckling mice and day-old chicks, and inoculation by the chorio-allantoic membrane, allantoic cavity and yolk sac of chick embryos. The most sensitive system for mouse-passaged Sindbis and MVE viruses was inoculation of 10-day-old chick embryos onto the chorio-allantoic membrane. Mouse-passaged Getah virus had no infectivity for chick embryos inoculated by the yolk sac or allantoic cavity, or for chickens inoculated intracerebrally, but focal lesions were produced on the chorio-allantoic membrane after inoculation by this route. The most sensitive system for this virus was the inoculation of mice intracerebrally. Egg-passaged Sindbis and MVE viruses gave results similar to those obtained with mouse-passaged viruses. Getah virus could not be recovered from the allantoic fluid after 5 blind passages by the allantoic cavity route in chick embryos and does not appear to multiply in this site.

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