Susceptibility of channel catfish (Ictalurus punctatus) fed with dietary sodium chloride to nitrite toxicity

Abstract

Juvenile channel catfish (Ictalurus punctatus) were fed with nutritionally complete, basal diets supplemented with NaCl at 0, 10, 20 or 40 g kg−1 diet (0, 1, 2, or 4%) to apparent satiation twice daily for 10 weeks. Catfish were exposed to nitrite after six (7.70 mg L−1 nitrite-N) and ten (7.18 mg L−1 nitrite-N) weeks of feeding to determine the effect of dietary NaCl supplementation on resistance to nitrite toxicity. Fish were sampled before (baseline, pre-exposure) and after 24-h nitrite exposure to determine the effects of dietary NaCl on haematology (haematocrit, haemoglobin and methaemoglobin) and plasma electrolyte dynamics (nitrite, chloride, sodium and potassium). Mortality from nitrite toxicity was also determined. Mortality from nitrite exposure tended to decrease with increasing NaCl in the diet at 6 weeks and was significantly lower in the 40 g kg−1 NaCl group (12.5%) compared to the control group (57.5%). A similar trend in mortality occurred at 10 weeks as well; however, the differences among dietary treatments were not significant. The improvements in blood MetHb (non-significant), chloride and nitrite levels in catfish may at least in part be responsible for the improved survival after nitrite exposure, which trended in support of the prevailing hypotheses for the positive effects of NaCl on nitrite toxicity.