Abstract
7153 Background: Survivin inhibits apoptosis and promotes mitosis. Its expression has been restricted to malignant tissues. High levels of survivin expression are associated with poor survival of cancer patients. Both nuclear and cytoplasmic staining of survivin was observed. However, the prognostic value of cellular compartmentation of survivin has been a subject of debate. Methods: Antisense oligonucleotides (ASO) against survivin were used to examine the impact of survivin inhibition upon mitosis, apoptosis and viability of H460 lung cancer cells. Immunohistochemistry was used to determine whether nuclear or cytoplasmic localization of survivin predicts survival of 48 patients with resected NSCLC. Results: The anti-Survivin ASO specifically attenuated the protein expression of survivin in H460 cells. Survivin inhibition using antisense oligonucleotides resulted in increases of apoptosis and mitotic arrests of H460 cells. H460 cells had significantly decreased viability following the treatment of the antisense oligonucleotides. Patients with nuclear staining of survivin had significantly shorter overall survival (relative risk: 3.9, p=0.02). Conclusions: Our data suggest that survivin is a potential therapeutic target and a prognostic biomarker for NSCLC. No significant financial relationships to disclose.
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