Abstract
BackgroundAs a known regulator of apoptosis, survivin has positive relationship with lymphatic metastasis in breast cancer. This study aims to detect the difference in expression between survivin and vascular endothelial growth factor-C (VEGF-C) in treated breast cancer cells and tissues, and to analyze the correlation among survivin, VEGF-C and lymphatic metastasis.MethodsPlasmid with survivin and VEGF-C shRNA and lentivirus with survivin gene were constructed and transfected into breast cancer cell ZR-75-30. Then the expressions of the two genes were examined using western blot analysis and real-time PCR. The change of invasiveness of breast cancer cells was assessed using matrigel invasion assay. Using immunohistochemistry, the expression of survivin and VEGF-C were analyzed in 108 clinical breast cancer cases with breast cancer tissue and lymph node.ResultsSurvivin regulated the expression of VEGF-C at both protein and mRNA levels in breast cancer cells. Immunohistochemical analysis showed that the level of VEGF-C expression was significantly related with that of survivin in breast cancer tissues (p<0.05). VEGF-C was found to participate in the process of breast cancer cells invasion mediated by survivin. The co-expression of the two and the single expression of any one took significant difference in positive lymph node (p<0.05).ConclusionsSurvivin takes an important part in regulating the expression of VEGF-C. VEGF-C could influence the invasive ability mediated by survivin. The co-expression of survivin and VEGF-C is more statistically significant to assess lymphatic metastasis in breast cancer.Virtual slidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/9193530897100952
Highlights
As a known regulator of apoptosis, survivin has positive relationship with lymphatic metastasis in breast cancer
Breast cancer was diagnosed and classified into various stages according to the International Union Against Cancer (UICC) and the TNM classification system published by the American Joint Committee on Cancer (AJCC)
Lentivirus-survivin with green fluorescent protein (GFP) was transfected into cells using the method mentioned above
Summary
As a known regulator of apoptosis, survivin has positive relationship with lymphatic metastasis in breast cancer. This study aims to detect the difference in expression between survivin and vascular endothelial growth factor-C (VEGF-C) in treated breast cancer cells and tissues, and to analyze the correlation among survivin, VEGF-C and lymphatic metastasis. More than half of breast cancers are associated with axillary nodal involvement. Many pathways have been suggested to be involved in the process of breast cancer lymphatic metastasis. Some markers, such as EGFR [3] and BCRP [4], could be detected as the predictable target of breast cancer lymphatic metastasis. Some researches have indicated that survivin and vascular endothelial growth factor-C (VEGF-C) may take part in the course of lymphatic metastasis of breast cancer
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