Survivin antisense oligonucleotide mediated by polyamidoamine dendrimer induces apoptosis of colorectal cancer cells

Abstract

Objective To investigate the possibility of polyamidoamine dendrimer for Survivin Antisense Oligonucleotide delivery system and explore the effects of polyamidoamine dendrimer-Survivin antisense oligonucleotide on the Survivin expression and apoptosis of human colorectal cancer cell line SW620. Methods The PAMAM anti-gene transfection complex and the liposome anti-gene transfection complex was manufactured. The shape of the complex were observed by transmission electron microscope, the size was determined by laser particle size analysator and the zeta potential was measured by analytical tool. The encapsulating efficiency, DNA loading level, release progress in vitro were determined by ultravio- let spectrophotometer in centrifuging method. The transfection efficiency of Survivin-asODN in SW620 cells was mearsured. The protein expression of Survivin was measured by Western blot analysis and the apoptosis rate of SW620 cells was assessed by flow cytometry. Results The diameter of PAMAM-Survivin-asODN complex was less than that of liPosome-Survivin-asODN complex(P < 0.01 ), the zeta potential was high than that for liposome-Survivin-asODN complex(P < 0.05 ). There was no significant difference between two groups for envelopment ratio and loaded dosage. The PAMAM' s release progress in vitro lasted 14 days and 5 days for the liposome. The transfection efficiency for PAMAM-Survivin-asODN was higher than that for liposome-Survivin-asODN (P < 0.05 ). The expression of Survivin protein for PAMAM-Survivin-asODN was less than that for liposome-Survivin-asODN(P <0. 05)and the cell apoptosis rate was higher than that for liposome-Survivin-asODN (P < 0. 01 ). Conclusion The polyamidoamine dendrimer can delivery Sur-vivin-asODN into SW620 cells effectively and induce SW620 cells apoptosis. Key words: Polyamidoamine dendrimer; Antisenze oligonucleotides (aSODN) ; Colorectal