SURVIVAL TIME AND HISTOLOGICAL OUTCOME OF MAJOR ORGANS FOLLOWING HONEY ADMINISTRATION TO ACUTE PARAQUAT-INTOXICATED RATS: A PRELIMINARY STUDY

Suk Peng Tang,Hasnan Jaafar,Siti Amrah Sulaiman,Siew Hua Gan,Kuttulebbai N.S Sirajudeen

doi:10.22159/ijpps.2017v9i2.15466

Suk Peng Tang, Hasnan Jaafar + Show 3 more

Open Access

https://doi.org/10.22159/ijpps.2017v9i2.15466

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Abstract

Objective: The objective of this research was to investigate the possible protective effect of Tualang honey (TH) in acute paraquat (PQ) toxicity in rats.Methods: A total of 48 male Sprague-Dawley rats aged eight weeks old were used. Oral PQ and TH were administered at 225 mg/kg and 0.2 g/kg, respectively. The effects of single and multiple TH treatmentson PQ-intoxicated rats were then investigated. Single TH treatment groups received TH at 0.5 (PQ+TH0.5h), 2 (PQ+TH2h) or 6 (PQ+TH6h) hours following PQ administration. Multiple TH treatment groups received TH at 0.5, 2 and 6 h (PQ+THtrp) or further daily treatment for the following six days (PQ+TH7d) after PQ administration (n=6 per group). The survival time of the rat was recorded until day 28 before sacrifice, which was followed by a histological examination.Results: Treatment with TH did not improve the survival rate of PQ-intoxicated rats. However, the median survival time of rats that received multiple TH treatments was significantly longer compared to that of the PQ+TH6h group. TH treatment was found to improve the histological outcomes of PQ-intoxicated rats, particularly in the lungs.Conclusion: Our findings suggest the potential role of honey in delaying the toxic effects of PQ.

Highlights

Paraquat (PQ) is a widely used herbicide, the challenges resulting from PQ exposure are reported all over the world and are mainly caused by suicidal intent, accidental poisoning or occupational exposures [1]
PQ is known to stimulate the production of various reactive oxygen species (ROS) via a single electron redox cycle in vivo[2, 3]
The conventional approach in treating PQ poisoning focuses on three main areas, which include the prevention of absorption from the gastrointestinal tract, the enhancement of elimination of PQ from the body and the administration of therapies directed against toxicity

Summary

Introduction

In comparing the groups that received only single honey treatments (at 30 min, 2 h or 6 h), there was a trend in which the earlier the animals were administered the honey treatment, the longer the observed median survival time. The death associated with multiple organ failures as observed in most cases of severe PQ poisoning was evidenced by post-mortem histological analyses of the lung, kidney and liver of rats, in the rats that died within four d≤ay9s6 (h) following PQ intoxication, with or without honey treatments.

Results

Conclusion