Survival Rates of Oryzias Celebensis Embryo Reared in Different Media in an Attempt to Provide Embryos for Ecotoxicological Studies

Abstract

Fish embryos from the genus Oryzias have long been used as animal models in ecotoxicological research. Research on the survival rate of Oryzias celebensis embryos in different rearing media has been carried out. In this study, the media used were embryo rearing medium (ERM), bottled water (BW), Pattunuang river water (PRW), and well water (WW). The data obtained is analyzed with a one-way ANOVA statistical test and descriptive analysis in the form of tables and figures. The results of this study indicated that the embryogenesis processes of O. celebensis in all media were faster than the development of O. latipes, which was used as a reference for embryogenesis observations. The yolk volume in each medium decreased in size along with the development of the embryogenesis phases. The results of the one-way ANOVA statistical test showed that the rearing medium was significantly different (P<0.05) concerning the hatching time parameter and not significantly different (P>0.05) on the parameters of survival rate of the embryo (SRe) and total larval length. The water quality of the rearing medium was still in a condition that the O. celebensis embryos could tolerate except for the concentration of CaCO3, which could affect the hatching time. This study concludes that embryo rearing medium (ERM) solution is the best medium for rearing O. celebensis embryos for ecotoxicological studies. The hatching time parameter of the O. celebensis embryo has the potential to be used as a biomarker in ecotoxicological studies.