Survival of Sclerotinia sclerotiorum Sclerotia in Central New York.

Abstract

White mold caused by Sclerotinia sclerotiorum is a serious disease affecting many field and specialty crops in New York (NY). The primary inoculum for white mold is sclerotia, which are hardened masses of mycelia that survive adverse environmental conditions and periods of nonhosts. However, NY crop guidelines lack rotation and residue management recommendations based on local knowledge of sclerotial survival. A field trial was established in October 2020 by deploying S. sclerotiorum sclerotia in mesh bags on the soil surface or shallowly buried (placed at a 3-cm depth in the soil) at Geneva, NY. Bags were periodically collected from 67 to 769 days. At each collection, sclerotial retrieval (number of sclerotia) was assessed by counting, and their viability was evaluated through myceliogenic germination. Sclerotial retrieval was significantly affected by soil depth and was higher in those on the surface than those buried. Time also affected the retrieval of sclerotia, which was significantly reduced after 250 days. The interaction between burial and time had a significant effect on sclerotial viability. Approximately 15% of sclerotia placed on the surface were still viable after 769 days. After 433 days, the viability of buried sclerotia was also significantly reduced compared to those on the surface. After 670 days, none of the buried sclerotia were viable. These findings suggest a rotation of at least 2 years between susceptible crops is required to reduce primary inoculum. However, given that low inoculum densities are sufficient to initiate a white mold outbreak, a longer rotation may be beneficial. In a cultivated system, timely tillage of crop residue to bury sclerotia after harvest to promote degradation is encouraged.