Abstract
The objective of this study is to verify potential of various types of microorganisms during spray drying and non-refrigerated storage that can be enhanced substantially by selecting suitable protective colloids. Four selected probiotics tested are Lactbacillus plantarum B13 and B18, which are the bacteria probiotics and Kluyveromyces lactis and Saccharomyces blouradii, non-bacteria probiotics. Two levels of experiment occur starting with formulation study of encapsulation agent followed by the viability study of different probiotics after spray dry and two weeks nonrefrigerated storage. The formulation of 30% of gum Arabic, 15% of gelatin and 45% of coconut oil can homogenize well at least for two hours and can produce acceptable dried product (below 4% of moisture content) at low outlet temperature (70℃ -75℃). K. lactis, S. blouradii gives 2.57% and 2.4% of viability percentage after spray drying process and 25.84% and 2.04% after two weeks nonrefrigerated storage respectively. The colonies of non-probiotics bacteria after both conditions are between 1010 and 106 cfu/mL which is among the accepted level for industrial application. However, the survival of probiotics in a spray-dried form during non-refrigerated storage is higher at low of moisture content compared to others.
Highlights
Probiotics have been recently defined as “live microbes which transit to the gastro-intestinal tract and having positive effects to the health for the consumers [1]
This study demonstrated the possibility of producing dry probiotic that suitable as an animal feed using spray drying
Using combination of gum Arabic, gelatin and coconut oil with inlet and outlet temperature, feed rate and solid percentage in the solution is 110°C, 75°C, 100 rpm and 13.5% respectively give the best powder form of product which is below 4%
Summary
Probiotics have been recently defined as “live microbes which transit to the gastro-intestinal tract and having positive effects to the health for the consumers [1]. It have been applied in aquaculture for controlling diseases, enhancing the immune response, supplementing or even in some cases replacing the use of anti microbial. Some of other probiotics which used in animal feed are microscopic fungi such as strain of yeasts. It was possible to apply yeast’s strain as probiotics strain in powder form of animal feed in line with the effectiveness in animal health. For example camel calves can improve weight gain, average daily gain and feed utilizations after apply yeast as supplementation diet in different forms [9]
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