Survival, growth and osmolality of greenlip abalone Haliotis laevigata (Donovan 1808) when exposed to different ionic profiles of inland saline water

Abstract

Two experiments over 21 days were conducted to assess the effect of different ionic profiles (mainly K+) of inland saline water (ISW) on the survival, growth, osmoregulation and moisture content of juvenile greenlip abalone Haliotis laevigata. In experiment I, the juveniles were cultured in five different water types: ocean water (OW) as a control, inland saline water (ISW or ISW K+0), ISW fortified with 33%, 66% and 100% K+ as in OW (ISW K+33, ISW K+66 and ISW K+100 respectively). In experiment II, five different culture media, 100% OW(control), 75% OW mixed with 25% ISW (ISW25), 50% OW mixed with 50% ISW (ISW50), 25% OW mixed with 75% ISW (ISW75) and 100% ISW (ISW or ISW100), were tested. Within 21 days, all of the abalone in ISW (ISW K+0 and ISW100; Na+/K+ ratio of 111 L−1) died compared with 100% survival in OW. One-hundred per cent K+ fortification of ISW increased the specific growth rate (SGR) and serum osmolality of the surviving juveniles. The 96 h acute LC50 value was between ISW50 and ISW75. The increased proportion of ISW in OW resulted in significantly (P<0.05) lower survival, SGR and serum osmolality of juveniles. The moisture content of the juveniles was not influenced by any water type. The results suggested that abalone cannot survive in raw ISW, but survival can increase when it is fortified with K+ and ionic ratios of ISW are brought closer to OW by mixing it with OW. Furthermore, abalone survival is influenced by other ions in ISW besides K+.