Abstract
Selected strains of ectomycorrhizal fungi can be inoculated in forest nurseries to improve survival and growth of seedlings or cuttings after field transplantation. The survival of the American strain Laccaria bicolorS238N on Douglas fir cuttings was evaluated in nursery and field conditions three years after outplanting using morphological and PCR/RFLP of nuclear rDNA spacers. The compari- son of the mycorrhizal status of Douglas fir cuttings at the end of the nursery phase and two years after outplanting shown several beha- viours among the ectomycorrhizal fungi naturally occurring in the nursery or artificially introduced. The naturally occurring Rhizopogon type disappeared after outplanting, while the inoculated strain Laccaria bicolorS238N and an unknown type (1/2 ITS ribotype) survived and competed with the naturally occurring fungi of the outplanting site. Only one indigenous type (1/3 ITS ribotype) seemed occurring in the outplanting site where Cenococcum geophilumwas almost completely absent.
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