Abstract
Survey of antibiotic treatment of Escherichia coli infection in broilers and efficacy of Enrofloxacin plus colistin in experimental Colibacillosis
Highlights
Introduction coli are worldwide in distribution and are Colibacillosis caused by E. coli is a disease found in intestinal tract of all warm blooded of significant economic concern in all species, found in the litter and fecal phases of poultry industry in Pakistan
At 28th day of age Group A was treated with Enrofloxacin plus Colistin Sulphate, group B with Enrofloxacin, group C with Colistin Sulphate and group D was kept as untreated control (Table 2)
Data collected from thirty E. coli infected farms showed that antibiotics in E. coli infection were mostly used in combination
Summary
Part - I Field Survey of antibiotic usage for treatment of E. coli Thirty E. coli infected poultry farms located in district Faisalabad were surveyed and following data was obtained. Part - II Isolation and identification of E. coli Livers and hearts of sick and dead birds of different age groups suffering from suspected E. coli infection were collected. Samples were transported to the laboratory and inoculated on MacConkey’s agar medium for primary isolation, purification and identification of E. coli from samples [13]. Morphology and staining reaction The morphological, cultural, staining characteristics, of isolates were determined. The ileum was cut and half milliliter culture of the test isolate was delivered into the lumen of the intestine. Preparation of inoculum After confirmation of the pathogenicity, E. coli isolate was inoculated into fresh nutrient broth and incubated at 37oC for overnight. At age of 26th day E. coli infection was induced by oral inoculation of 1mL of 1×106 cfu/bird in all groups.
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