Abstract
Objective The aim was to determine the effects on red blood cell (RBC) and plasma lipid peroxidation, antioxidants and blood hematology of intraperitoneally administered vitamin E (VE) and 1% methylene blue (MB) solutions for prevention of adhesions in rats. Study design Thirty-seven female Sprague–Dawley rats were randomized into four groups. An adhesion model was constituted on the left uterine horn in three of the groups. They were then given intraperitoneally 0.9% saline (C group), 10 mg VE (VE group) and 1% MB (MB group) solutions, respectively. A sham group (Sh group), on which laparotomy was performed, received 2 ml of 0.9% saline solution. Results In the C group, the adhesion scores were significantly higher than in the VE ( p < 0.01), MB ( p < 0.01) and Sh groups ( p < 0.005). Results showed that adhesion formation significantly induced nitric oxide (NO) ( p < 0.01) and malondialdehyde (MDA) in plasma ( p < 0.01). The levels of RBC glutathione (GSH) ( p < 0.05) and plasma VE ( p < 0.01) significantly increased after VE administration, whereas the levels of MDA (RBC and plasma) ( p < 0.01), plasma NO ( p < 0.01), blood lymphocyte count ( p < 0.05) and blood white blood cell (WBC) counts ( p < 0.01) decreased. Treatment with MB caused a significant increase in plasma VE ( p < 0.01). On the other hand, results showed that MB significantly decreased blood WBC counts ( p < 0.01), plasma paraoxonase (PON1) ( p < 0.001) and NO ( p < 0.01), and RBC glutathione peroxidase (GPx) activity ( p < 0.05) and MDA levels ( p < 0.01). Conclusion Intraperitoneal administration of MB and VE is significantly effective in preventing intraabdominal adhesion formation in a rat model. Further investigations are necessary, however, to better understand the underlying biochemical mechanisms on lipid peroxidation and antioxidants of MB.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
