Abstract

Blocking protein-protein interactions (PPI) using small molecules or peptides modulates biochemical pathways and has therapeutic significance. PPI inhibition for designing drug-like molecules is a new area that has been explored extensively during the last decade. Considering the number of available PPI inhibitor databases and the limited number of 3D structures available for proteins, docking and scoring methods play a major role in designing PPI inhibitors as well as stabilizers. Docking methods are used in the design of PPI inhibitors at several stages of finding a lead compound, including modeling the protein complex, screening for hot spots on the protein-protein interaction interface and screening small molecules or peptides that bind to the PPI interface. There are three major challenges to the use of docking on the relatively flat surfaces of PPI. In this review we will provide some examples of the use of docking in PPI inhibitor design as well as its limitations. The combination of experimental and docking methods with improved scoring function has thus far resulted in few success stories of PPI inhibitors for therapeutic purposes. Docking algorithms used for PPI are in the early stages, however, and as more data are available docking will become a highly promising area in the design of PPI inhibitors or stabilizers.

Highlights

  • Rational drug design has revolutionized the pharmaceutical industry with the idea that drugs can be designed or engineered according to an identified protein or DNA target

  • Since different docking programs developed by different researchers around the world use different criteria for scoring based on the need and the problem encountered, a general assessment method for results of docking was established to compare the quality of docked protein complex structures

  • Docking methods are used in various stages of protein-protein interactions (PPI) inhibitor design whether it is small molecule-based or peptide-peptidomimetic-based drug design

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Summary

Introduction

Rational drug design has revolutionized the pharmaceutical industry with the idea that drugs can be designed or engineered according to an identified protein or DNA target. In other words, designed compounds have to be optimized for drug-like properties in three-dimensional space This type of optimization necessitates knowledge of the 3D structures of the protein receptors involved as well as the conformational space of the ligand drug [1]. With detailed information about the binding site of a protein receptor available, computational methods such as docking have gained importance in optimizing drug-like compounds. Such computational methods were established in the 1980s [2] for the drug discovery process; there were several limitations. In most cases, searching methods are time-consuming, and searching for all possible conformations and orientations of a molecule on the receptor surface is an impossible task

Protein-Protein Interactions
Identification of Hot Spots on Proteins and Docking in PPI
Method
Identification of Hot Spots and Druggability
Examples of Design of PPI Inhibitors
Conclusions
Findings
Conflicts of Interest
Full Text
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