Surfce Functionalized via AdLAMA3 Multilayer Coating for Re-epithelization Around Titanium Implants.

Jing Zhang,Yuan Shi,Jialu Li,Jinxing Hu,Guoli Yang,Yongzheng Li

doi:10.3389/fbioe.2020.00624

Jing Zhang, Yuan Shi + Show 4 more

Open Access

https://doi.org/10.3389/fbioe.2020.00624

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Abstract

The peri-implant epithelium (PIE) forms a crucial seal between the oral environment and the implant surface. Compared with the junctional epithelium (JE), the biological sealing of PIE is fragile, which lacks hemidesmosomes (HDs) and internal basal lamina (extracellular matrix containing laminin332, IBL) on the upper part of the interface. In the study, we aim to prepare a coating with good biocompatibility and ability to immobilize the recombinant adenovirus vector of LAMA3 (AdLAMA3) for promoting the re-epithelization of PIE. The titanium surface functionalized with AdLAMA3 was established via layer-by-layer assembly technique and antibody-antigen specific binding. The biological evaluations including cell adhesion and the re-epithelization of PIE were investigated. The results in vitro demonstrated that the AdLAMA3 coating could improve epithelial cell attachment and cell spreading in the early stage. In vivo experiments indicated that the AdLAMA3 coating on the implant surface has the potential to accelerate the healing of the PIE, and could promote the expression of laminin α3 and the formation of hemidesmosomes. This study might provide a novel approach and experimental evidence for the precise attachment of LAMA3 to titanium surfaces. The process could improve the re-epithelization of PIE.

Highlights

Dental implants have become important and popular prosthodontic devices for replacing missing teeth due to caries or periodontal disease in clinic (Hong and Oh, 2017)
The use of dental implants to rehabilitate the loss of teeth has markedly increased in the last 30 years (Jenny et al, 2016), peri-implantitis is a significant complication of dental implants that is caused by local factors such as oral bacterial infection, systemic factors such as nutritional status, hormonal abnormality and hematologic disorder (Reynolds, 2014; Holtfreter et al, 2015; Lee et al, 2018)
These results indicated that the coatings fabricated by the LBL technique improved the cytocompatibility of the native titanium surface, while there was no difference among the three coating groups

Summary

Introduction

Dental implants have become important and popular prosthodontic devices for replacing missing teeth due to caries or periodontal disease in clinic (Hong and Oh, 2017). The titanium has been considered the standard material for the treatment of edentulous jaws (Nishihara et al, 2019). The use of dental implants to rehabilitate the loss of teeth has markedly increased in the last 30 years (Jenny et al, 2016), peri-implantitis is a significant complication of dental implants that is caused by local factors such as oral bacterial infection, systemic factors such as nutritional status, hormonal abnormality and hematologic disorder (Reynolds, 2014; Holtfreter et al, 2015; Lee et al, 2018). Peri-implantitis is considered to be the major cause of implant failure. The biological sealing between the implant and adjacent soft tissue is closely related to the initiation of peri-implantitis (Ramanauskaite and Juodzbalys, 2016; Schwarz et al, 2018).

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