Surfactant protein D as additional marker in diagnostics of asthma and its combination with gastroesophageal reflux disease with pulmonary manifestations

Abstract

Considering the concept of M1/M2 programming macrophages can obtain pro-inflammatory M1 or anti-inflammatory M2 phenotype and change the phenotype in the disease formation. One of significant regulators of macrophages activity is surfactant protein D (SP-D). Objective: Assessment of SP-D level and oligomeric forms in BALF in patients with asthma (A), gastroesophageal reflux disease (GERD) with pulmonary manifestations and A+GERD combination vs. healthy volunteers (HV). Methods: SP-D level in BALF was assessed by ELISA (BioVendor, κaT. N o 194-0591). Oligomeric SP-D forms were analysed by Western blot analysis (tris-acetate gels, Invitrogen, NuPAGE, # EA03752BOX). Results: SP-D level in BALF in A patients was 1.80 times increased and in GERD - 2.66 times decreased vs patients with combination of A and GERD (748.32±69.25 ng/ml, 155.83±18.13 ng/ml vs 414.72±50.22 ng/ml, p Conclusion: In asthma, GERD with pulmonary manifestations and their combination quantitative and qualitative composition of SP-D in BALF vary against healthy volunteers and each other. In asthma patients SP-D level was increased vs HV. GERD was characterized by maximum decreased SP-D level. Oligomeric forms of SP-D in asthma were similar to HV. In GERD and asthma+GERD oligomeric SP-D composition was significantly changed vs HV but with no significant differences between the groups.