Abstract
This review incorporates a large number of chromatographic systems modified by the surfactants. A large number of solvent systems and stationary phases are summarized in this paper. Three different kinds of surfactants (anionic, cationic, and nonionic) are used as modifiers for stationary phases as well as solvent systems. Surfactants are used at all the three different concentration levels (below, above, and at critical micelle concentration) where surfactants behave differently. Modifications of both stationary phases and solvent systems by surfactants produced a new generation of chromatographic systems. Microemulsion solvent systems are also incorporated in this paper. Microemulsion thin-layer chromatography is a new approach in the field of chromatography.
Highlights
It is a well-known fact that amino acids are building blocks of proteins
This review article deals with the surfactant modified thin-layer chromatographic systems used for the analysis of amino acids
Surfactants are the class of compounds which entirely modify the efficiencies of different phases involved in the chromatography
Summary
It is a well-known fact that amino acids are building blocks of proteins. Amino acids are essential ingredients of diets of all living beings. Because of the enormous applications of amino acids in many biological systems chromatographic study of these molecules is very important. This review article deals with the surfactant modified thin-layer chromatographic systems used for the analysis of amino acids. The most exciting features found in MTLC as compared to conventional thin-layer chromatography that leads to new generation of chromatographic systems are (a) double solvent front that is observed in chromatogram, (b) modifications of adsorbent surfaces, and (c) a change in elution order of compounds [3]. The use of aqueous micellar mobile phases is free from some disadvantages such as strong smell, volatility, flammability, Journal of Analytical Methods in Chemistry aggressiveness, and toxicity [3] as compared to the organic solvents used in conventional TLC. Because the use of surfactants in MTLC leads to the generation of eco-friendly chromatographic systems, MTLC has tremendous applications in the field of separation science. Micellar phase has been widely used for the identification and separation of various pharmaceuticals, vitamins, amines, metal ions, and many other closely related compounds [4,5,6,7,8,9,10,11,12,13]
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