Abstract
The application of surface-enhanced resonance Raman scattering (SERRS) spectroscopy to the analysis of the configuration of biliverdin dimethyl ester (BVDE) is reported. SERRS spectra obtained by adsorption of the compounds onto an electrochemically roughened silver electrode and recorded at 7 K were intense and free of significant photodegradation. The similarity of the SERRS and resonance Raman (RR) spectra obtained under identical conditions suggests that no perturbation of the electronic structure of the BVDE occurs upon interaction with the silver surface, and that the distribution of conformers comprising the BVDE solution is not changed. SERRS spectra of the deuterated and monoprotonated Z,Z,Z isomer are also presented. To investigate the influence of configuration upon the Raman spectrum we have synthesized and purified the E,Z,A, and Z,Z,E isomers of BVDE. Excellent SERRS spectra were obtained from the solutions of the compounds eluted directly from the TLC plates.
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