Abstract
Packed fused-silica columns with several surface pretreatments are prepared for micro liquid chromatography. Untreated fused silica is compared to polymethylhydorsiloxane-deactivated tubing as well as a polymer-coated commercially available capillary GC column. Columns are packed under identical conditions with 5-μm reversed-phase material. The chromatographic and physical properties of these columns are examined. Surface pretreatments have minimal effects on the chromatographic characteristics of packed fused-silica columns but substantial effects on the physical capabilities.
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