Surface Tension, Spinnability, and Gelation of Denatured Carp Actomyosin Preparation.

Abstract

Actomyosin preparation was treated by denaturating agents. When NH2C(=NH)NH2HSCN was added to actomyosin preparation in water or 0.6M KCl, surface tension and spinnability showed the maximum around 3M, decreased at 6M and decreased farther at 8M. When CO(NH2)2 was added to actomyosin preparation, surface tension and spinnability showed the maxima at 2M and around 8M. It was suggested from the great changes of surface tension and spinnability that structure change (unfolding) of actomyosin was induced with the treatments by these denaturating agents. When actomyosin preparation in water or 0.6M KCl was treated by NH2C(=NH)NH2HSCN or CO(NH2)2 and dialysed against water to remove the denaturating agent, 6M-treatment pro-duced firm gel, 4M-treatment produced medium gel, and 8M-treatment produced soft gel. It was concluded that the local unfolding of actomyosin molecule by 6M-treatment was more favoured for the gelation of actomyosin than the complete unfolding by 8M-treatment. When actomyosin preparation was treated by NH2C(=NH)NH2HSCN and dialysed against 0.6M KCl in place of water, the gel made by 6M-treatment was softer than the gels made by 4M- and 8M-treatments.