Abstract
The surface elemental composition of Balb 3T3 A31 HYF mouse fibroblast cells and transformed MSV/MLV Balb 3T3 A31 HYF cells was obtained using x-ray photoelectron spectroscopy (XPS). The surface composition of the cells was sensitive to the nature of the specimen preparation; however, the oxygen to nitrogen (O/N) atom ratio varied between 3 and 1 for washed cells and fixed cells. The oxygen to nitrogen atom ratio was generally lower for transformed cells. Cell coverage on glass substrate was estimated to be 95% by comparing the silicon 2p signal from clean glass and glass substrate overlaid with cells. The change in concentration of phosphorus and sodium to a depth of approximately 40 nm into the surface of the cells was obtained combining XPS surface analysis data and oxygen plasma etching. The change in phosphorus concentration was linear with time and fairly similar for the cells before and after transformation.
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