Abstract
An improved method of preparing two-dimensional surface spreads of synaptonemal complexes (SCs) in higher plants for examination by electron microscopy is described. This protocol produces clear, well-spread preparations of SCs and unpaired axial cores from a range of meiotic prophase I stages (leptotene to pachytene) from meiocytes of different plant species. Synaptonemal complex (SC) analyses have been widely used in plant cytogenetic studies to address the process of meiotic chromosome synapses, because of the high-resolution allowed by electron microscopy. Although the real role of SC is still enigmatic, its presence and structural conservation in the vast majority of organisms reflect the importance of this protein structure in the meiotic process.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
