Surface properties of plasma membrane vesicles isolated from melon ( Cucumus melo L.) root cells differing in salinity tolerance

Abstract

The hypotheses that genotypic differences in salinity tolerance may result from (i) differences in global surface charge density or (ii) from differences in global Ca 2+ binding were tested. An attempt was made to correlate the differing salinity tolerance of four melon cultivars with surface properties of vesicles extracted from the plasma membrane (PM) of their root cells. Surface characterization involved measurements of electrophoretic mobility and sorption of 45Ca 2+ to the vesicles in the presence of varying concentrations of Ca 2+, Na + and Mg 2+. Irrespective of salinity tolerance, vesicles from the four cultivars yielded similar ζ potentials under similar conditions, indicating similar global surface charge densities. Sorption studies with vesicles from two cultivars differing in salinity tolerance predicted independently this result of equal surface charge density. The estimated global binding affinities of Ca 2+, Na + and Mg 2+ to the PM of both cultivars were the same with binding coefficients of 50, 0.8 and 9 M −1, respectively. Consequently, the hypotheses enumerated above to interpret genotypic differences in salinity toxicity are rejected. However, vesicles from the salt-resistant strain sorbed 19% more Ca 2+ per given amount of protein in the membrane, indicating the existence of a larger number of negatively charged surface sites per given amount of protein and a smaller amount of protein per given area of membrane. Genotypic differences in site-specific Ca 2+-binding affinity (e.g. at ion channels) remain a viable hypothesis for genotypic differences in salinity tolerance.