Surface properties and early murine pre-osteoblastic cell responses of phosphoric acid modified titanium surface

Abstract

AimsThe present study investigated the surface properties and murine pre-osteoblast cell (MC3T3-E1) responses of phosphoric acid (H3PO4) treated commercially pure titanium. MethodsTitanium discs were treated with various concentration of H3PO4 (5%, 10%, and 20%; v/v) at 90°C for 30min. Surface properties were evaluated by profilometer, contact angle meter, and scanning electron microscopy (SEM) with energy dispersive X-rays. MC3T3-E1 attachment and spreading were evaluated by SEM and phalloidin immunohistochemistry staining. ResultsSurface roughness and wettability were not statistically difference among all experimental and control groups. Phosphate and oxygen were detected on H3PO4 treated surfaces. At 20min, cell attachment was significantly higher in 10% and 20% H3PO4 treated groups compared to the control. Cells exhibited orientated-cytoskeleton fibers on 20% H3PO4 modified titanium surface. Though, there was no difference in cell spreading stage among all treatment groups. ConclusionH3PO4 treatment on titanium may influence early cell response, particularly on attachment and spreading.