Abstract
Abstract The optoelectronic biosensor, based on surface plasmon resonance (SPR) for detection of photosynthesis-inhibiting pesticides in aqueous solutions was presented. The pesticide capability to replace plastoquinone from its complex with D1 protein was used for the detection. This replacement reaction results in the changes of the optical characteristics of protein layer, immobilized on the gold surface. Monitoring of these changes with SPR-technique permits to determine 0.05–5.0 μg/ml pesticide in solution within 1 h.
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