Abstract
A method for reconstructing three-dimensional images of thin films from surface plasmon resonance (SPR) microscopy images is described. As an example, monolayers of a lipid were deposited onto a gold substrate using Langmuir–Blodgett technology and an SPR microscope image obtained. Using data from reflectance-angle (R-θ) curves obtained in supplementary measurements, the contrast in the microscope image was used to provide thickness information. Converted to a z modulation this was used to produce a three-dimensional image of the monolayer.
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