Surface plasmon resonance imaging biosensor based on silicon photodiode array

Abstract

The detection limit of surface plasmon resonance imaging (SPRI) biosensor is constrained in part by the SPR biochip and in part by the resolution of the optical intensity of detecting instruments. In this paper, silicon photodiode is proposed as the optical intensity detecting element instead of the traditionally used charge coupled device (CCD), combining with high resolution analog/digital converter, this method can efficiently reduce the cost and increase the sensitivity of the SPRI system while keeping its virtue of multiple channels real time detecting. Based on this method, An SPRI experimental system with two channels is designed and the optical intensity of each channel is detected by a photodiode. By carrying out testing experiments using sucrose solution with different concentrations (corresponding to different refractive index), the system sensitivity of 10-6 refractive index unit (RIU) is obtained.