Surface Plasmon Resonance Imagery-based strategy to study hnRNPA2/B1 peptides as specific biomarkers in systemic lupus erythematosus, rheumatoid arthritis and autoimmune hepatitis

Abstract

Event Abstract Back to Event Surface Plasmon Resonance Imagery-based strategy to study hnRNPA2/B1 peptides as specific biomarkers in systemic lupus erythematosus, rheumatoid arthritis and autoimmune hepatitis Elvire Beleoken1, 2, 3*, Herve Leh4, Eleonora De Martin3, Catherine Johanet1, 2, 5, Didier Samuel1, 2, 3, Jean-Charles Duclos-Vallee1, 2, 3, Malcolm Buckle4 and Eric Ballot1, 2, 5 1 Inserm, Unite 785, France 2 Univ Paris-Sud, Unité Mixte de Recherche en Santé 785, France 3 AP-HP Hopital Paul Brousse, France 4 Institut d'Alembert, Laboratoire de biologie et pharmacologie appliquée (LBPA), France 5 AP-HP, Hôpital Saint Antoine, France Heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 is one of antinuclear autoantibodies targets in systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), and autoimmune hepatitis (AIH). Aim. To monitor molecular interactions between peptides spanning the entire sequence of hnRNP and sera from patients and healthy controls. Methods. Sera from 8 patients from each pathology and controls were passed across a Surface Plasmon Resonance Imagery (SPRi) surface containing 39 overlapping peptides of 17 mers covering the human hnRNP B1. Interactions involving the immobilised peptides were followed in real time and the dissociation rate constant koff for each interaction were calculated. Results. Several significant interactions were observed: i) high stability (lower koff values) between P55-70 and the AIH sera compared to controls (p= 0.003); ii) less stability (higher koff values) between P118-133 and P262-277 and SLE sera, P145-160 and RA sera compared to controls (p=0.006, p=0.002, p=0.007). The binding curves and koff values observed after the formation of complexes with anti-IgM and anti-IgG antibodies and after nuclease treatment of the serum indicate that i) IgM isotypes are prevalent and ii) nucleic acids participate in the interaction between anti-hnRNAP B1 and P55-70 and also between controls and the peptides studied. Conclusions. These results indicate that P55-70 of hnRNP B1 is a potential biomarker for AIH in immunological tests and suggest the role of circulating nucleic acids, (eg miRNA), present or absent according to the autoimmune disorders and involved in antigen-antibody stability. Keywords: Autoimmune Hepatitis, SPRi (surface Plasmon resonance imagery), heterogeneous nuclear ribonucleoproteine A2/B1(hnRNP A2/B1), biomarkers, miRNA Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune receptors and signaling Citation: Beleoken E, Leh H, De Martin E, Johanet C, Samuel D, Duclos-Vallee J, Buckle M and Ballot E (2013). Surface Plasmon Resonance Imagery-based strategy to study hnRNPA2/B1 peptides as specific biomarkers in systemic lupus erythematosus, rheumatoid arthritis and autoimmune hepatitis. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00102 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Mrs. Elvire Beleoken, Inserm, Unite 785, Villejuif, France, documentaliste.chb@pbr.aphp.fr Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Elvire Beleoken Herve Leh Eleonora De Martin Catherine Johanet Didier Samuel Jean-Charles Duclos-Vallee Malcolm Buckle Eric Ballot Google Elvire Beleoken Herve Leh Eleonora De Martin Catherine Johanet Didier Samuel Jean-Charles Duclos-Vallee Malcolm Buckle Eric Ballot Google Scholar Elvire Beleoken Herve Leh Eleonora De Martin Catherine Johanet Didier Samuel Jean-Charles Duclos-Vallee Malcolm Buckle Eric Ballot PubMed Elvire Beleoken Herve Leh Eleonora De Martin Catherine Johanet Didier Samuel Jean-Charles Duclos-Vallee Malcolm Buckle Eric Ballot Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.