Abstract
In this paper, we demonstrate bio-sensing response of bio-medically relevant proteins by employing a surface plasmon resonance (SPR)-based experimental setup that uses a set of double prisms in Kretschmann configuration. The SPR signal of nanoscale Ag-films of varying thicknesses was first monitored experimentally and then verified through computational simulation. The SPR signal was found to be substantially modulated at the metal–dielectric interface when the nanoscale Ag-films are exposed to bio-molecules, such as catalase, papain, bovine serum albumin, trypsin, and lysozyme, which were evaluated independently.
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