Abstract
In this study, we designed a simple, rapid, sensitive and selective surface plasmon resonance (SPR) sensor for detection of L-phenylalaine by utilizing molecular imprinting technology. l-phenylalanine imprinted and non-imprinted poly(2-hydroxyethyl methacrylate-methacryloyl-l-phenylalanine) polymeric films were synthesized onto SPR chip surfaces using ultraviolet polymerization. l-phenyalanine imprinted and non-imprinted SPR sensors were characterized by using contact angle, atomic force microscopy and ellipsometry. After characterization studies, kinetic studies were carried out in the concentration range of 5.0–400.0 μM. The limit of detection and quantification were obtained as 0.0085 and 0.0285 μM, respectively. The response time for the test including equilibration, adsorption and desorption was approximately 9 min. The selectivity studies of the l-phenylalanine imprinted SPR sensor was performed in the presence of d-phenylalanine and l-tryptophan. Validation studies were carried out via enzyme-linked immunosorbent analysis technique in order to demonstrate the applicability and superiority of the l-phenylalanine imprinted SPR sensor.
Highlights
L -phenylalanine ( L -Phe), which is an aromatic and hydrophobic amino acid, is effective in the treatment of brain diseases as it can cross the blood–brain barrier.L -Phe is the precursor amino acid of L -tyrosine ( L -Tyr) and is involved in several proteinrelated activities [1,2,3]
The surface morphologies of surface plasmon resonance (SPR) chip surfaces were characterized with atomic force microscobe (AFM) in the tapping mode
The results showed that the L-Phe imprinted (MIP) SPR sensor had higher adsorption capacity for
Summary
L -phenylalanine ( L -Phe), which is an aromatic and hydrophobic amino acid, is effective in the treatment of brain diseases as it can cross the blood–brain barrier.L -Phe is the precursor amino acid of L -tyrosine ( L -Tyr) and is involved in several proteinrelated activities [1,2,3]. For the diagnosis of PKU disease, phenylalanine, known as an indicator substance in the diagnosis of PKU disease, has different methods, including the amount of plasma determining Guthrie test, microbial inhibition, fluorimetric, chromatography and mass spectrometry [13,14,15,16]. These methods are time consuming and expensive, insufficient sensitivity, complex instrumentation, special laboratory facilities and highly experienced labor force requirements limit their use in routine analysis. Fast and economical methods of determination are needed for the diagnosis of the PKU disease
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