Surface Plasmon Resonance-Based Immunosensor for Igm Detection with Gold Nanoparticles.

Aykut Arif Topçu,Monireh Bakhshpour,Nilay Bereli,Adil Denizli

doi:10.3390/mi12091092

Aykut Arif Topçu, Monireh Bakhshpour + Show 2 more

Open Access

https://doi.org/10.3390/mi12091092

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Journal: Micromachines	Publication Date: Sep 10, 2021
Citations: 10	License type: CC BY 4.0

Affiliation: Aksaray University, Hacettepe University

Abstract

In this work, a surface plasmon resonance (SPR) based immunosensor was prepared by the immobilization of the amine-functionalized gold nanoparticles (N-AuNPs) on the sensing surface to sense immunoglobulin M (IgM) antibodies in the aqueous solution and artificial plasma. The characterization studies of SPR based immunosensor for IgM detection were performed with scanning electron microscope (SEM), contact angle measurements, and ellipsometry. Kinetic studies for the IgM immunosensor were carried out in the range of 1.0 to 200 ng/mL IgM concentrations in an aqueous solution. The total IgM analysis time including adsorption, desorption, and regeneration cycles was nearly 10 min for the prepared immunosensor. The limit of detection (LOD) and limit of quantification (LOQ) were found as 0.08 and 0.26 ng/mL, respectively. The reusability of the proposed immunosensor was tested with 6 consecutive adsorption-desorption, and regeneration cycles. Also, enzyme-linked immunosorbent assay (ELISA) method was utilized in the validation of the immunosensor.

Highlights

enzyme-linked immunosorbent assay (ELISA) is a well-established and highly selective method used in clinical studies depended on the antigen-antibody interactions; the requirement of the labelled molecule, the cost, and the long incubation time are the major drawbacks of ELISA
surface plasmon resonance (SPR) based sensors measure a change in the refractive index at the sensing surface depending on analyte concentrations and this optical phenomenon allows to investigate of the binding kinetics of the molecules [37]
Before the design of a SPR sensor, the receptor is immobilized on a sensing surface and after that, the kinetic analysis of the target molecule is investigated in real time and without a need of labelled molecule

Summary

Introduction

Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. ELISA depended on the colorimetric method by using the enzyme-labelled antibodies is a widely adopted sensitive approach for the detection of antibodies [2,10]; the long incubation time, the need for multi-washing step, and the requirement of the labelled molecules are the main disadvantages for practical use of ELISA in clinical trials To overcome these limitations, SPR based sensors measuring the change in the refractive index near the sensing surface with real-time and label-free monitoring have been attracted more attention of the scientists because of the higher sensing capability of the various molecules including, the clinical analyte of interest [11,12,13]. G (IgG) and hemoglobin (Hb) as competitor molecules in the aqueous solution, and the feasibility of the immunosensor was investigated in the artificial plasma

Materials

Preparation of SPR Based Immunosensor

Characterization Studies

Real-Time Monitoring of IgM

Selectivity and Reusability Studies

Sensing Method

Findings

Conclusions