Abstract
Surface plasmon resonance detection was used to characterize interactions of human topoisomerase I and DNA. The disassociation of topoisomerase I and DNA is characterized by two rate constants. This suggests two parallel but independent pathways of release. DNA association appears to be complex. Mg2+ was found to increase both disassociation rate constants and may have a detectable effect on topoisomerase I-DNA association. DNA base content did not affect disassociation rate constants or the rate of association.
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