Abstract

AbstractA surface plasmon is a charge density wave propagating along the interface between a metal on dielectric medium. Since the energy is concentrated at the interface, sensors that utilize the surface plasmon are extremely sensitive to both the charges in the dielectric properties and the geometry of the interface.A surface plasmon immunosensor to detect antibody and antigen immunoresponse has been developed. Here the sensitivity of the sensor is theoretically investigated and the experimental results of sensor sensitivity, the real‐time measurements and the problem of repeated utilization of the sensors are evaluated using α‐Feto‐protein (α‐FP) and goat IgG. In addition, the problem of deciding whether to use antibody or antigen as immobilized sensing medium is discussed based on the characteristics of surface plasmon and biomolecules. As a result, it is concluded that with an antigen immobilized sensor, the sensitivity is higher and the real‐time response can be detected.

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