Surface physico-chemistry governing microbial cell attachment and biofilm formation on coal

Abstract

An essential but missing component in our understanding of microbial interactions leading to coal bed methane (CBM) formation lies in the relationship between coal and coal-adhering microorganisms. Here, we explored the influence of surface physico-chemistry on the microbial cell attachment on coal, utilising a known coal-oxidising bacterium P. fluorescens Pf-5 as a model. Based on the interfacial free energy of adhesion calculation and direct observation of cell adhesion on coal, natural lignite and subbituminous coals offered the most conducive surface for cell attachment driven by favourable hydrophobic and acid-base interactions. Coal pretreatments dramatically decreased coal surface hydrophobicity and increased its electron-donating potential (γ−), resulting in unfavourable interaction with cells that were hydrophilic and possessed high γ−. An exception to this was peroxide treatment, which maintained a low γ− of the coal surface and allowed favourable coal-cell interaction to occur. Cell colonisation on lignite appeared to be the strongest amongst all coal types, likely due to the relatively high van der Waals surface energy component that influence the adhesion strength in favourable cell-coal interactions. This would partly explain the higher methanogenic potential found in lignite compared to subbituminous coal in previous studies, as more adsorbed cells potentially lead to greater liberation of small organic compounds. Although our cell enumeration results generally correlated with the thermodynamic data, some exceptions were present that signify the biological role in cell-to-coal attachment and biofilm formation. Overall, these findings provide insights into the fundamental parameters that are involved in cell-coal adhesion dynamics at the molecular level.