Abstract
Objective To evaluate the surface morphology and in vitro leachability of temporary soft linings modified by the incorporation of antifungals in minimum inhibitory concentrations (MIC) for Candida albicans biofilm.Methodology Specimens of soft lining materials Softone and Trusoft were made without (control) or with the addition of nystatin (Ny), miconazole (Mc), ketoconazole (Ke), chlorhexidine diacetate (Chx), or itraconazole (It) at their MIC for C. albicans biofilm. The surface analyses were performed using Confocal laser scanning microscopy after 24 h, 7 days, or 14 days of immersion in distilled water at 37ºC. In vitro leachability of Chx or Ny from the modified materials was also measured using Ultraviolet visible spectroscopy for up to 14 days of immersion in distilled water at 37ºC. Data (μg/mL) were submitted to ANOVA 1-factor/Bonferroni (α=0.05).Results Softone had a more irregular surface than Trusoft. Morphological changes were noted in both materials with increasing immersion time, particularly, in those containing drugs. Groups containing Chx and It presented extremely porous and irregular surfaces. Both materials had biexponential release kinetics. Softone leached a higher concentration of the antifungals than Trusoft (p=0.004), and chlorhexidine was released at a higher concentration than nystatin (p<0.001).Conclusions The surface of the soft lining materials changed more significantly with the addition of Chx or It. Softone released a higher concentration of drugs than Trusoft did, guiding the future treatment of denture stomatitis.
Highlights
Studies in vivo have reported that the nocturnal wear increases the colony counts of C. albicans, which reinforces that such habit can induce denture stomatitis
At 24 h, they presented irregular surfaces exhibiting pearls and small pores distributed on the surface (Figures 3A and 4A)
Surface change with the increase in immersion time was observed in both materials, especially in those containing drugs, in which extremely porous and irregular surfaces were observed for groups containing chlorhexidine diacetate and itraconazole; 2
Summary
Denture-induced stomatitis is considered the most common fungal infection among denture wearers. This pathology is primarily associated with infection by Candida albicans, which is found in 50 to 98% of all cases. Treatments for denture stomatitis are varied and include topical and systemic antifungal therapy, oral hygiene care, procedures for denture cleaning and disinfection, replacement of old dentures, removal of anatomical irregularities, reestablishment of nontraumatic occlusion, and nutritional restitution. to protect and preserve the mucosal integrity, the patients should sleep without the dentures. Studies in vivo have reported that the nocturnal wear increases the colony counts of C. albicans, which reinforces that such habit can induce denture stomatitis.6Topical antifungal agents are widely used in the therapy for this condition. their effectiveness can be compromised by many factors, including lack of patient perception of the infection, costs required for the medication, continuous denture wear, unpleasant taste, and patient compliance in strictly following the posology. salivary flow, tongue movements, and swallowing decrease drug concentration to subtherapeutic doses. systemic administration of antifungal agents should be carefully administered these drugs can induce hepatotoxic and nephrotoxic effects.. Denture-induced stomatitis is considered the most common fungal infection among denture wearers.. Denture-induced stomatitis is considered the most common fungal infection among denture wearers.1 This pathology is primarily associated with infection by Candida albicans, which is found in 50 to 98% of all cases.. Topical antifungal agents are widely used in the therapy for this condition.. Topical antifungal agents are widely used in the therapy for this condition.1 Their effectiveness can be compromised by many factors, including lack of patient perception of the infection, costs required for the medication, continuous denture wear, unpleasant taste, and patient compliance in strictly following the posology.. Systemic administration of antifungal agents should be carefully administered these drugs can induce hepatotoxic and nephrotoxic effects.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
