Abstract
We introduce a method that combines two-photon polymerization (TPP) and surface functionalization to enable the indirect optical manipulation of live cells. TPP-made 3D microstructures were coated specifically with a multilayer of the protein streptavidin and non-specifically with IgG antibody using polyethylene glycol diamine as a linker molecule. Protein density on their surfaces was quantified for various coating methods. The streptavidin-coated structures were shown to attach to biotinated cells reproducibly. We performed basic indirect optical micromanipulation tasks with attached structure-cell couples using complex structures and a multi-focus optical trap. The use of such extended manipulators for indirect optical trapping ensures to keep a safe distance between the trapping beams and the sensitive cell and enables their 6 degrees of freedom actuation.
Highlights
Mechanical manipulation of micrometer and nanometer scale biological objects has become a significant issue in the bio-nanotechnology field during the last decade
In this paper we show that 3D microstructures made of SU-8 photopolymer by two-photon polymerization (TPP) can be functionalized effectively and in order to use for indirect optical micromanipulation of single cells
In the case of specific streptavidin binding there is no significant difference between the UV- and the TPP polymerized SU-8 surface, both reaching about 4·104 molecules/μm2 density
Summary
Mechanical manipulation of micrometer and nanometer scale biological objects has become a significant issue in the bio-nanotechnology field during the last decade. A further important motivation for indirect manipulation is to avoid photo- or thermal damage the intense optical field may impose on the sensitive biological matter, cells in particular [18,19,20]. While the use of microbeads is well-established, it is limited to motions only with 3 degrees of freedom and it still brings the trapping field close to the trapped object. To overcome these limitations, the use of extended, designed intermediate objects is a promising alternative
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