Surface modification of polystyrene microspheres with synthetic antigenic determinants of human immunodeficiency virus

Abstract

The chemisorption of synthetic antigenic determinants of the human immunodeficiency virus on the surface of polystyrene microspheres with surface layers containing polystyrene-poly(vinylpyrrolidone) graft copolymers and carboxyl groups has been studied. The electrosurface properties of carrier particles before and after modification with peptides and agglutination of peptide-modified latexes with hyperimmune rabbit antisera have been investigated. It has been demonstrated that the stabilization of the structure of the hydrophilic surface layer via lengthening of polystyrene chains in the graft copolymers leads to an increase in the efficiency of exposure of antigenic determinants at the interface and improves the sensitivity of latex immunoreagents produced on their basis.