Abstract
The use of micro- and nanodevices as multifunctional systems for biomedical applications has experienced an exponential growth during the past decades. Although a large number of studies have focused on the design and fabrication of new micro- and nanosystems capable of developing multiple functions, a deeper understanding of their interaction with cells is required. In the present study, we evaluated the effect of different microparticle surfaces on their interaction with normal and tumoral human breast epithelial cell lines. For this, AlexaFluor488 IgG functionalized polystyrene microparticles (3 μm) were coated with Polyethyleneimine (PEI) at two different molecular weights, 25 and 750 kDa. The effect of microparticle surface properties on cytotoxicity, cellular uptake and endocytic pathways were assessed for both normal and tumoral cell lines. Results showed a differential response between the two cell lines regarding uptake efficiency and mechanisms of endocytosis, highlighting the potential role of microparticle surface tunning for specific cell targeting.
Highlights
On the other hand, micro- and nanoparticles cellular uptake and the mechanism of internalization can differ among cell types[12,13,18,26]
More research is required to improve the strategies for biomedical applications of micron-sized delivery systems, as they have been proved to be a good approach for drug delivery[31,32,33,34,35]
The use of micro- and nanosystems for drug delivery has shown a strong potential for biomedical applications, a deeper understanding of their interaction with cells is required for more efficient design of drug delivery systems
Summary
Micro- and nanoparticles cellular uptake and the mechanism of internalization can differ among cell types[12,13,18,26]. Specific antibodies or peptides against certain cell surface markers are used to enhance particle internalization in target cells[27,28,29,30]. The aim of the present work was to provide an integrated study about the impact of different non-specific surface modifications of microparticles upon their interaction with different cell types, in terms of cytotoxicity, uptake efficiency, mechanism of internalization and intracellular fate. With this purpose, 3-μ m polystyrene microparticles were functionalized with a fluorescently-labeled non-specific antibody. The cytotoxicity and internalization of those microparticles were studied in two human breast epithelial cell lines, one normal and another tumoral
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