Surface modification of graphene oxide with stimuli-responsive polymer brush containing β-cyclodextrin as a pendant group: Preparation, characterization, and evaluation as controlled drug delivery agent.

Abstract

In this work, stimuli-responsive graphene oxide/polymer brush nanocomposites (GPBNs) prepared through the polymerization of acrylic acid (AA), N-isopropylacrylamide (NIPAM) and acrylated β-cyclodextrin (Ac-β-CD) from the graphene oxide (GO) surface. The attachment of polymers on the GO surface was approved using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), UV-vis spectroscopy (UV-vis) and thermogravimetric (TGA) analysis. Scanning electron microscopy (SEM) was used to observe the morphological change on the GO surface after polymer grafting. Transition electron microscopy (TEM) showed that polymeric brushes were decorated on the GO surface. The growth of polymer brushes on the GO was further confirmed using atomic force microscopy (AFM). Both hydrophilic (doxorubicin, DOX) and hydrophobic (Methotrexate MTX) drugs were co-loaded in the prepared graphene Oxide/Polyacrylated β-cyclodextrin/polyacrylic acid/poly N-isopropylacrylamide brush nanocomposite (GCANBN). Drug releases from GCANBN were studied using UV-vis. MTT assay was used for the evaluation of in-vitro cytotoxicity of GCANBN. The prepared system showed its efficacy as a nanocarrier for both types of drugs.