Abstract
Quantum dots (QDs) are powerful materials in various bioapplications based on their excellent optical and electronic properties. For the application of various fields of QDs, surface modification of QDs is necessary. However, surface modification in QDs may result in a reduction in quantum yield (QY). This reduction of QY causes many weaknesses in the biological application of QDs. In this study, CdSeZnS/ZnS alloy QDs were used to prepare antibody-conjugated QDs for a sandwich immunoassay. The alloy QDs displayed a QY of 84.5% that was maintained at 83.0% (98.2% of QY was maintained) after surface modification with the anti-rabbit IgG as a model study. Surface-modified QDs successfully detected their corresponding target through antibody-antigen binding. The limit of detection was 1.1 × 10 2 ng mL-1 for rabbit IgG.
Highlights
Semiconductor quantum dots (QDs) are increasingly being used in both fundamental and applied research because of their optical and electronic properties [1,2,3]
The method of depositing a secondary shell after the primary refining core was used in the core synthesis method of the alloy QDs
The ligand of the primary shell in the synthetic method was applied with a mixture of various thiol-contained ligands (1-octanethiol, 1-dodecanethiol, 1-decanrthiol...) which reduced the lattice mismatch in the response to increase the stability of the core in the alloy QDs
Summary
Semiconductor quantum dots (QDs) are increasingly being used in both fundamental and applied research because of their optical and electronic properties [1,2,3]. QDs can absorb a broad range of light and emit the specific wavelength of light which depends on their size. With this property, one of the useful applications of QDs is fluorescent probes in biological imaging [4]. QDs are dispersed only in nonpolar solvents and limited with severe intermittency in emission. Their nonspecific binding can lead to misinterpretation of experimental results. The critical issue of QDs is toxicity which can cause acute or chronic diseases Another issue of QDs is their instability in biological media because of their high hydrophobic property. Surface modification is required to make QDs which have hydrophilic characteristic and more stable in a biological environment [7,8,9,10,11,12,13,14]
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