Abstract
Purpose: Artificial valves have disadvantages that they cannot grow, repair and remodel. Tissue engineered heart valves are expected to overcome these shortcomings. The durability of tissue-engineered heart valves is now questionable. The purpose of the study is to evaluate of the durability of tissue engineered heart valve with surface modified scaffolds. Method: The scaffold was constructed with poly(D, L lactid-co-glycol-ide) (PGLA). To enhance cell attachment and in-growth, surface properties were modified by immobilization (if fibronectin and adhesion peptide sequence (RGD). For effective surface modification, the scaffolds were CO2 gas plasma treated prior to the immobilization process. The mock circulation system using a pulsatile pump was used for durability test under various shear stresses physiological condition. The cell-polymer construct was evaluated with MTT assays. In addition, scanning electron microscopy (SEMI study of the seeded scaffolds was also performed to document tissue development. Result: MTI assay and SEM evaluation revealed that in surface modified scaffolds, cell attachment was enhanced and cells were well ingrown to he tissue like structure. consequently the result of durability tests showed that the cells on the tissue engineered heart valve scaffolds with modified surface endured the diverse physiological shear stress Acknowledgement: This work was supported by the BK 21 project.
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