Surface grafting of chitosan shell, polycaprolactone core fiber meshes to confer bioactivity

Abstract

Electrospinning of polyesters (e.g. polycaprolactone) is an attractive approach for fabricating meshes with mechanical properties suitable for orthopedic tissue engineering applications. However, the resultant fused-fiber meshes are biologically inert, necessitating surface grafting of bioactive factors to stimulate cell adhesion. In this study, hydrophilic meshes displaying primary amine groups were prepared by coaxially electrospinning fibers with a chitosan/poly(ethylene oxide) shell and a polycaprolactone core. These chitosan–polycaprolactone fiber meshes were mechanically robust (Young’s modulus of 10.1 ± 1.6 MPa under aqueous conditions) with tensile properties comparable to polycaprolactone fiber meshes. Next, the integrin adhesion peptide arginine–glycine–aspartic acid was grafted to chitosan–polycaprolactone fiber meshes. Cell culture studies using bone marrow stromal cells indicated significantly better initial attachment and spreading on arginine–glycine–aspartic acid–conjugated fiber meshes. Specifically, metabolic activity, projected cell area, and cell aspect ratio were all elevated relative to cells seeded on polycaprolactone and unmodified chitosan–polycaprolactone meshes. These results demonstrate a flexible two-step process for creating bioactive electrospun fiber meshes.