Surface glycoprotein patterns of B type chronic lymphocytic leukaemia cells correlate with the clinical activity of the disease.

Abstract

The surface glycoprotein patterns of leukaemic B lymphocytes from 20 patients with clinically progressive or non-progressive chronic lymphocytic leukaemia (CLL) were investigated. Cells were labelled by the neuraminidase-galactose oxidase-tritiated sodium borohydride technique and the radioactive proteins were separated by polyacrylamide slab gel electrophoresis and visualized by fluorography. A total of 13 to 16 bands were detected. A common surface glycoprotein pattern for CLL cells was seen in all patients consisting of 7 proteins with the apparent molecular weights of 210, 200, 185, 150, 135, 110 and 90 kilodaltons, respectively. Interesting differences were, however, observed as cells from patients with progressive CLL in general lacked the glycoproteins 120, 72 and 67 K, which were found on cells from inactive CLL. The possible biological and clinical significance of these findings is discussed.