Abstract
BackgroundMinocycline has been widely used in central nervous system disease. However, the effect of minocycline on the repairing of nerve fibers around dental implants had not been previously investigated. The aim of the present study was to evaluate the possibility of using minocycline for the repairing of nerve fibers around dental implants by investigating the effect of minocycline on the proliferation of Schwann cells and secretion of neurotrophic factors nerve growth factor and glial cell line-derived neurotrophic factor in vitro.MethodsTiO2 nanotubes were fabricated on the surface of pure titanium via anodization at the voltage of 20, 30, 40 and 50 V. The nanotubes structure were characterized by scanning electron microscopy and examined with an optical contact angle. Then drug loading capability and release behavior were detected in vitro. The TiO2 nanotubes loaded with different concentration of minocycline were used to produce conditioned media with which to treat the Schwann cells. A cell counting kit-8 assay and cell viability were both selected to study the proliferative effect of the specimens on Schwann cell. Reverse transcription-quantitative PCR and western blot analyses were used to detect the related gene/protein expression of Schwann cells.ResultsThe results showed that the diameter of TiO2 nanotubes at different voltage varied from 100 to 200 nm. The results of optical contact angle and releasing profile showed the nanotubes fabricated at the voltage of 30 V met the needs of the carrier of minocycline. In addition, the TiO2 nanotubes loaded with the concentration of 20 μg/mL minocycline increased Schwann cells proliferation and secretion of neurotrophic factors in vitro.ConclusionsThe results suggested that the surface functionalization of TiO2 nanotubes with minocycline was a promising candidate biomaterial for the peripheral nerve regeneration around dental implants and has potential to be applied in improving the osseoperception of dental implant.
Highlights
IntroductionThe effect of minocycline on the repairing of nerve fibers around dental implants had not been previously investigated
Minocycline has been widely used in central nervous system disease
These nerve regeneration were important to implant osseoperception, defined as the sensation arising from mechanical stimulation of a bone-anchored prosthesis, transmitted by mechanoreceptors that may include those located in bone, joint, mucosal and periosteal tissues
Summary
The effect of minocycline on the repairing of nerve fibers around dental implants had not been previously investigated. It suggested that formation of a titanium implant surface with a nanostructure could reinforce osseointegration because the surface area is markedly increased and the surface topography can be nano modified to resemble native bone tissue. Lots of studies focus on improving bone regeneration around implants and osseointegration between implants and bone tissue, researches on nerve regeneration around dental implants are rare. Whereas, these nerve regeneration were important to implant osseoperception, defined as the sensation arising from mechanical stimulation of a bone-anchored prosthesis, transmitted by mechanoreceptors that may include those located in bone, joint, mucosal and periosteal tissues. The improvement of nerve regeneration around implants to enhance the sensory perception of the implant-supported denture is of clinical significance
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