Abstract
Boron doped diamonds (BDD) are a promising material for fabrication of neuroelectrodes and formation of neurointerfaces. Surface properties of BDD thin films can be adjusted by variation of grain sizes, patterning, lithography techniques and coating by different biopolymers to further promote performance of neuroelectrode and enhance the neuron adhesion.We used 3T3 fibroblasts and rat hippocampal neurons (newborn and adult) to estimate planar and nanostructured BDD surface biocompatibility. Optical microscopy, lactate dehydrogenase assay and calcium imaging were used to check cell adhesion and longterm survival.3T3 fibroblasts are able to grow on BDD without any coating. No signs of cytotoxicity and changes in cell cycle were found. Adhesion and longterm survival of newborn rat hippocampal neurons were observed only on poly‐L‐lysine coated planar and nanostructured BDD surface. Calcium imaging of longterm cultures of newborn neurons grown on poly‐L‐lysine coated planar and nanostructured BDD showed functionality and synaptic activity of hippocampal neurons. Nanodiamond surface without poly‐L‐lysine coating do not support adhesion of newborn neurons.Adult rat hippocampal neurons preferred poly‐D‐lysine coated planar nanodiamonds but were able to grow on nanostructured diamonds without any coating.Support or Funding InformationThis work was supported by Czech Science Foundation grant 17–15319S.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
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